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KMID : 0358920180450010032
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Journal of the Korean Academy of Pedodontics
2018 Volume.45 No. 1 p.32 ~ p.40
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Study of Bacteria Associated with Dental Caries Using a 3 Tone Disclosing Agent
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Lee Jeong-Eun
Park Ho-Won
Lee Ju-Hyun
Seo Hyun-Woo
Lee Si-Young
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Abstract
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The aim of this study was to determine the efficacy of a 3 tone plaque disclosing gel in assessing the risk of caries related to the population of Streptococcus mutans , Streptococcus sobrinus , and Lactobacillus spp . quantified using a quantitative real-time polymerase chain reaction (qRT-PCR). 15 healthy children of ages 9 - 12 years were randomly examined. The 3 tone plaque disclosing gel was applied on teeth surfaces, which changed the color to pink or red, blue or purple and light blue. Plaque was divided into 3 groups based on staining. Genomic DNA from each sample was subjected to a qRT-PCR assay for quantitative detection of target bacteria. The Kruskal-Wallis test was conducted for correlation between the color of plaque and the number of bacterial species. The levels of S. mutans , S. sobrinus , and Lactobacillus spp . were significantly different in the plaque samples of the 3 groups (p < 0.05). The proportion of S. sobrinus to S. mutans showed correlation to the color of plaque. The different color-dyed plaque was related to the number of acidogenic bacteria. The 3 tone plaque disclosing gel could be used as one of the indicators to assess the clinical risk of caries associated with the population of S. mutans , S. sobrinus , and Lactobacillus spp.
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KEYWORD
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3 Tone disclosing agent, Dental plaque, Quantitative real-time polymerase chain reaction, Streptococcus mutans, Streptococcus sobrinus, Lactobacillus spp.
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